Johannes Kepler (1571–1630) – mathematician, astronomer, and astrologer. Collaborated with
Tycho Brahe. Imperial mathematician of Rudolph II, in Prague.
Wolfgang Amadeus Mozart (1756–1791)– composer; Don Giovanni was first performed on 29 October 1787 in Prague. Amadeus Mozart himself conducted the world premiere.
Carl Maria von Weber (1786–1826) – composer and conductor, director of the Estates Theatre, Prague
Gerstner, František Jozef (1759-1832) – Designed the first horse-drawn railroad on the European continent
Antonín Dvořák (1841–1904) – composer,director of the Prague Conservatory
Albert Einstein (1879–1955) – physicist, professor at Charles University in Prague. Einstein published eleven of his works during his stay in Prague.
Christian Doppler (1803-1853) – professor of mathematics and practical geometry at the High Polytechnic School; in Prague, he discovered and formulated the famous physical effect now known as the Doppler Effect
Franz Kafka (1883–1924) – one of the most significant and globally acclaimed writers of the of the 20 century: The Metamorphosis and Other Stories, The Trial, etc.
Jaroslav Heyrovský (1890–1967) – chemist and inventor, born in Prague, professor at the Charles University Faculty of Science. Heyrovský was the inventor of the polarographic method, father of the electroanalytical method, and laureate of the Nobel Prize in 1959.
Vladimir Prelog (1906-1998) – Nobel prize laureate in chemistry in 1975. Studied Chemistry at the Czech Institute of Technology (now the University of Chemistry and Technology)
Miloš Forman (1932) – Czech film director, screenwriter, actor, and professor. Studied in Prague.
Václav Havel (1936-2011) – writer, philosopher, and statesman, born in Prague. From 1989 to 1992, he served as the first democratically elected president of Czechoslovakia.